Find datasets with sgRNA similar to yours

Examples :  GACGCAGATAAGAACCAGTT,   GTATCCTCTATGATGGGAGA,   CTTGACCAATAGCCTTGACA,   GAACGACTAGTTAGGCGTGTA



Predict efficiency for your sgRNA

Examples :  TCCCTCTATGTGCTCGAAGCAGG GCTGCAGGGCCTTTGCTGGGCGG
Notes
  • Selecting the model type of your purpose and its corresding sequence with a right NGG, the CRISPRa model should be 23bp (20bp sgRNA sequences with an NGG PAM) and the CRISPRoff model input sequence should be 40bp (9bp upstream and 8bp downstream sequences together with the 23bp target sequence).
  • If you want to use the epigenetic model, you need to input the corresponding chromosome position and gene information.